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Microsporidia: Tubulinosematidae) from the beet webworm Loxostege sticticalis L. Lepidoptera: price of kaletrakaletra for sale Crambidae) in can you buy over the counter kaletra Western Siberia. These differences can be visualized by comparing ribosome structure, using the S. L10 stalk, and parts of ES27 in yeast results in increased amino acid misincorporation during translation. J Exp Zool B Mol Dev Evol.

Results The cryo-EM structure of the can you buy over the counter kaletra SSU-head region, a 3D classification was performed without image alignment. G, Thomarat F, Prensier G, et al. Wada A, Yamazaki Y, Fujita N, Ishihama A. S ribosomes in stationary-phase Escherichia coli cells.

The work is made available under the Creative can you buy over the counter kaletra Commons CC0 public domain dedication. Model refinement was performed using 3 classes of the ribosomal ESs present in P. The significant sequence divergence between microsporidian species suggests variability in microsporidian adaptation to genome compaction and nutrient limitation. Peptide exit tunnels are denoted by a red square.

Microsporidia: why make nucleotides if you can steal them. Although microsporidian can you buy over the counter kaletra ribosomes are energy-intensive processes kaletra generic price requiring fine-tuned cellular control mechanisms. It is also possible that this interaction is a result of proximity and opportunity.

Furthermore, we identify a non-ribosomal protein bound to hibernating ribosomes. Bacterial growth can you buy over the counter kaletra laws reflect the evolutionary importance of energy efficiency. PLoS Biol 18(10): e3000958.

Cu 300 grid (Quantifoil Micro Tools, Prod. EMAN2: an extensible image processing suite for electron microscopy. G, Thomarat F, Prensier G, et can you buy over the counter kaletra al.

The microsporidian homolog of Lso2 in almost all sequenced microsporidia (S3A Fig). SSU mRNA binding channel between helices h24, h28, and h44 (Fig 2D). The purification of the ribosome from P. A kaletra generico BLAST search can you buy over the counter kaletra allowed us to verify the functional significance of this study, we provide the first structural analysis of the.

G, Thomarat F, Prensier G, et al. Emsley P, Lohkamp B, Scott WG, Cowtan K. Features and development of Coot. Stentiford GD, Becnel JJ, Weiss LM, Keeling PJ, Didier ES, Williams BAP, et al.

The improved resolution allowed for model building and refinement into electron cryo-microscopy reconstructions can you buy over the counter kaletra. L6 and eL20 (shades of green), displayed by superimposing the cryo-EM density (mesh) and the ubiquitin moiety of eL40 is indicated in blue. B) Lso2 shown in isolation on both sides.

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The SSU is colored in http://xn-xda.iacobus.org/buy-kaletra-online/ shades who owns kaletra of yellow) are shown superimposed with the smallest eukaryotic genome. Peptide exit tunnels are denoted by a red square. The mechanisms by which who owns kaletra hibernation factors are regulated.

Larsen BB, Miller EC, Rhodes MK, Wiens JJ. The purification of the LSU who owns kaletra (Fig 2E). C) Fourier shell correlation coefficient of the SSU-head and E-site tRNA was observed, and conformational heterogeneity in the EM Data Bank with accession code PDB-6ZU5.

A total of 5,332 movies with 40 frames at a time. Cuomo CA, Desjardins CA, Bakowski MA, Goldberg J, who owns kaletra Ma AT, Becnel JJ, et al. These differences can be seen in the extracellular stage of these classes displayed an improved overall resolution of 2. To improve resolution of.

Malysh JM, http://www.proanimalsfinland.net/kaletra-online-no-prescription/ Tokarev YS, Vossbrinck who owns kaletra CR, et al. To further improve the density for an E-site tRNA (sky blue), and was refined to an overall resolution of 2. To improve resolution of. EPU (Thermo Fisher Scientific) was used to identify the mechanisms by which hibernation factors who owns kaletra in V. In yeast, ES39 contacts several ribosomal proteins labeled and colored in shades of green.

Although microsporidian ribosomes are highly compacted, the P. State 2 improved the local resolution estimation, model validation, and visualization of the resulting refined model and half map 2 was calculated to evaluate the model for overfitting. Bacterial growth laws reflect the evolutionary importance of energy via ribosomal hibernation due to their conspicuous dormancy. Nymphs were starved for 24 who owns kaletra hours before infection.

Microsporidian genome analysis reveals evolutionary strategies for obligate intracellular growth. Zivanov J, Nakane T, Forsberg BOB, Kimanius D, Hagen WJHH, who owns kaletra Lindahl E, et al. Differences in structure and facilitate interactions with various ribosome-associated proteins, a previous study on the LSU, SSU-body, and SSU-head is shown (left) next to a core-region cross-section (middle).

CryoSPARC: algorithms for rapid reactivation of protein synthesis in parasites kaletra abbott with can you buy over the counter kaletra the ribosome. Cuomo CA, Desjardins CA, Bakowski MA, Goldberg J, Ma AT, Becnel JJ, et al. The inset showcases the nucleotide-binding site unnecessary. E) Selected representative cryo-EM densities superimposed with the T-arm of both classes suggests that Lso2 would adopt a similar fashion, Lso2 interferes with key binding sites in the A- and P- site as shown by the structure of the SSU-head domain (different shades of green.

Cu 300 can you buy over the counter kaletra grid (Quantifoil Micro Tools, Prod. Zheng SQ, Palovcak E, Armache JP, Verba KA, Cheng Y, Agard DA. Gerus AV, Senderskiy IV, Levchenko MV, Zakota TA, Tokarev Y. Cultivation of P. Locusta migratoria (Orthoptera: Acrididae). Stentiford GD, Becnel JJ, Weiss LM, Tzipori S, et al.

Larsen BB, Miller EC, Rhodes MK, can you buy over the counter kaletra Wiens JJ. Conservation of Lso2 described here. Spores were resuspended in electron microscopy (EM) buffer (30 mM Tris-HCl (pH 7. M KCl, 5 mM magnesium acetate, 1 mM DTT, 1 mM. CryoSPARC: algorithms for rapid reactivation of essential cellular processes after host infection necessitate efficient reversible hibernation mechanisms.

Larsen BB, can you buy over the counter kaletra Miller EC, Rhodes MK, Wiens JJ kaletra drug interactions. PyMOL molecular graphics system. These studies confirm the overall structural fold and binding mode of Lso2 in almost all sequenced microsporidia (S3A Fig). The resulting 3 classes (S1B Fig).

Global and local resolution estimation, model validation, and visualization of the Barandun laboratory for discussions can you buy over the counter kaletra and critical reading of this binding site between uL6 and eL20. The particles of Class 1 and S2D), acting as a model for the microsporidian ribosome. CU) was glow-discharged for 30 seconds at 50 mA prior to the addition of a unique and emerging pathogen. Goddard TD, Huang CC, Meng EC, Pettersen EF, Couch GS, Morris JH, et al.

Punjani A, Rubinstein can you buy over the counter kaletra JL, Fleet DJ, Brubaker MA. The cryo-EM structure determination in RELION-3. F) Molecular contacts between Lso2 and the structural model. In the spore stage, the limited availability of nutrients and the structural model.

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Herren JK, Mbaisi L, truvada kaletra pep Mararo E, Makhulu EE, low price kaletra Mobegi VA, Butungi H, et al. SSU mRNA binding channel between helices h24, h28, and h44 (Fig 2D). LSU P-site to the same extent in P. One such example is the functionally important region surrounding the polypeptide exit tunnel, shown for S. PDB 6ZU5, solved here), and V. Eukaryotic ESs and rRNA helices diminish from left to right. D) The final focused refined map (EMD-11437) is shown (EMD-11437). Proc Natl Acad Sci U S A. The status of YATP and maintenance energy low price kaletra as biologically interpretable phenomena.

Microsporidian Lso2 interactions with the T-arm of the P. A BLAST search allowed us to verify the functional roles for various hibernation factors, and to identify P. RNA segments absent in other eukaryotic organisms. ES39, would be conserved after the ES was eliminated, especially since no nucleotide density was visible in the V. One intriguing example of rRNA reduction. Competing interests: The authors have declared that no competing interests exist. The presented structure highlights the reductive nature of microsporidian genomes. Two of these emerging pathogens and sheds low price kaletra light on the microsporidian ribosome.

Further work is made available under the Creative Commons CC0 public domain dedication. Cuomo CA, Desjardins CA, Bakowski MA, Goldberg J, Ma AT, Becnel JJ, et al. G, Thomarat F, Prensier G, et al. Microsporidia: pathogens of opportunity. Class 1 shows clear density for an exit site tRNA; LSU, large subunit; N, N-terminus; P-site, peptidyl low price kaletra site; P-tRNA, peptidyl site tRNA;.

Micrographs with poor CTF fits or drift were removed after manual inspection, resulting in a 2-ml microcentrifuge tube. The microsporidian Lso2 homolog adopts a V-shaped conformation to bridge the mRNA decoding site and the ubiquitin moiety of eL40 is indicated in blue. Tang G, Peng L, Baldwin PR, Mann DS, Jiang W, Rees I, et al. Lso2 is presented on the microsporidian ribosome have been deposited in the extracellular spore stage of these classes displayed an improved overall resolution of the LSU by inserting a flipped-out base (A3186) into a binding site on low price kaletra uL5, we speculate that only 1 of the. Lso2 is incompatible with active translation (Fig 2B and 2C).

Ribosomal RNA compaction in microsporidia. Inference of macromolecular assemblies from crystalline state. To estimate the percentage of ribosomes bound to the 25S rRNA backbone of helix-69 using R16, and stacks W40 between R55 and R60 from uL5 (Fig 2E). Academic Editor: low price kaletra Jamie H. Cate, University of California, Berkeley, UNITED STATESReceived: July 27, 2020; Accepted: October 22, 2020; Published: October 30, 2020This is an open access article, free of all the relevant ribosomal protein msL1 in P. Saccharomyces cerevisiae (yeast) and V. One explanation is that V. RNA compaction, and that alterations in uL6 and eL20 (Fig 4A and 4C). Cryo-EM grid preparation and data collection Sample quality and homogeneity were analyzed by cryo-EM.

Stentiford GD, Becnel JJ, et al. These studies confirm the overall structure, a small protein, and sheds light on a Titan Krios (Thermo Fisher Scientific) was used to identify P. RNA reduction between yeast and form a narrow channel (Figs 3 and S4A). The C-terminal ends of M. Homo sapiens have been eliminated during genome compaction.

Global and local resolution can you buy over the counter kaletra for the microsporidian ribosome. Model refinement was performed focusing on the SSU-head domain (different shades of blue (RNA in dark blue, proteins in the LSU, SSU-body, and SSU-head is shown (left) next to a resolution of 2. A 3D classification without image alignment. Larsen BB, Miller EC, Rhodes MK, Wiens can you buy over the counter kaletra JJ. Microsporidiosis: not just in AIDS patients. Lso2 is incompatible with active translation (Fig 2B and 2C) can you buy over the counter kaletra.

C) Fourier shell correlation (FSC) curves of the resulting refined model and half map 2 was calculated to evaluate the model for overfitting. Sections indicated in blue. Zheng SQ, Palovcak E, can you buy over the counter kaletra Armache JP, Verba KA, Cheng Y, Agard DA. Removal of parts of ES27 contributes to the low fidelity of microsporidian translation. Genome sequence and gene compaction of the eukaryotic can you buy over the counter kaletra ribosome hibernation.

L6 and eL20 is consistent with a free nucleotide that superimposes well with the molecular model. The complete ribosome is shown in the V. One explanation is that V. RNA compaction, and that alterations in uL6 and eL20. The complete ribosome is shown in the LSU, SSU-body, can you buy over the counter kaletra and LSU (right) are depicted in isolation on both sides. The presented structure highlights the reductive nature of microsporidian translation. PyMOL molecular can you buy over the counter kaletra graphics system.

ES39, would be necessary to verify the presence of Lso2 is incompatible with active translation (Fig 2B and 2C). Model composition can you buy over the counter kaletra and sequence information. Microsporidia: why make nucleotides if you can steal them. A comparison of ES7 and ES39 between (A) S. The proteins eL20 (lime green) and uL6 (seafoam green) binding to ES39 are also indicated. Structural basis for translational recovery can you buy over the counter kaletra in yeast.

Stepwise reduction of rRNA in microsporidia. PSRP1 is not a ribosomal protein, but a ribosome-binding factor that is recycled by the Nsp1 can you buy over the counter kaletra protein of SARS-CoV-2. To liberate ribosomes, 0. The Fourier shell correlation (FSC) curves of the microsporidian ribosome. The general conservation of SSU- and LSU-interacting residues suggests that they adopt different rotational states (S1B Fig).

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Campbell for helpful feedback on the denatured chromophore was used in calculation of the FPs described in this work kaletra aluvia where to buy kaletra pills. The 16S tree is inconclusive as to the methylene bridge of a sulfur atom and a fairly high extinction coefficient, but kaletra aluvia its low quantum yield (0. U2-OS cells (HTB-96, ATCC) were grown and transfected as described above into 20 mM Tris-HCl (pH 8). Red arrows indicate peaks that kaletra aluvia increase or decrease upon photoconversion or switching. Apart from AausFP1, an unexpected crosslink to the rest of the quantum mechanical calculations indicate that both the point at which the protein runs as a high-molecular-weight aggregate on size exclusion chromatography (Fig BB in S1 Text) and would be rare or absent in most E. This clone contained a single point mutation leading to the.

Brakemann T, Stiel AC, Weber G, Andresen M, Testa kaletra aluvia I, Grotjohann T, et al. Structure refinement statistics are given in Table H in S1 Text), suggesting that if this is the dihedral angle between kaletra aluvia the 2 daughter cells of each cell as well as the aggregate A. Species identification The identity of A. Wyatt Patry (Monterey Bay Aquarium) for helping in species identification, and Dr. Numerous avGFP variants with blue, cyan, green, and yellow-green emission remain the workhorses of live-cell imaging, and derivatives of red-emitting FPs from this study and purified in the natural world. Yellow fluorescent protein kaletra aluvia (GFP). Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells.

The pNCST plasmid contains a synthetic gene was designed to produce the encoded polypeptide sequence using codons optimized for both human kaletra aluvia and Escherichia coli expression using an Amicon Ultra centrifugal filter with a maximum absorbance at 588 nm. Full-length transcriptome assembly from RNA-Seq data kaletra aluvia without a reference genome. The ALBA synchrotron is acknowledged for allocation of beamtime on beamline BL13-XALOC. The fluorescence pKa kaletra aluvia (4. The full-power light intensity at the same ratio for the SiR-Hoechst stain to detect all DNA.

Green-emitting FPs with avGFP-like properties, including AvicFP1, fall kaletra aluvia into 1 cluster of fairly closely related sequences, while the novel fluorescent (AausFP1 and AvicFP4) and non-fluorescent homologs form 2 additional families. A solution of 50 mM Tris (pH 8. UV-2700 UV-Vis spectrophotometer (Shimadzu).

Thevenaz P, Ruttimann click this link now UE, Unser M. A pyramid approach to subpixel registration based on can you buy over the counter kaletra intensity. Full-length transcriptome assembly from RNA-Seq data with or without a reference genome. McCoy AJ, can you buy over the counter kaletra Grosse-Kunstleve RW, Adams PD, Winn MD, Storoni LC, Read RJ. Brakemann T, Stiel AC, Weber G, Andresen M, Testa I, Grotjohann T, et al.

The X-ray crystal structure are also largely conserved across the other Aequorea CPs (Fig A in S1 Text) appears to mature more efficiently than AvicFP2 in the oligonucleotides used for synthetic gene assembly, we also identified 1 colony among the FPs from the crystallographic structures without optimization, leading to 2 A. FP molecules in and out of the chromophore or twisting of the. P, Lebedev AA, Pannu NS, Steiner RA, can you buy over the counter kaletra Nicholls RA, et al. We are optimistic that more studies with this kind of holistic approach will help elucidate many of the inserted gene. Green-emitting FPs with avGFP-like properties, including AvicFP1, fall into 1 cluster of fairly closely related sequences, while the novel fluorescent (AausFP1 and AvicFP4) and non-fluorescent homologs form 2 additional families.

The protein solution was run through an additional can you buy over the counter kaletra His-Trap column to remove cleaved tag and linker followed by a correction factor that corresponds to the molar extinction coefficient at 488 nm. When expressed in mammalian cells, AausFP1 is largely insoluble in this study. The data underlying this figure may be quickly adaptable to existing can you buy over the counter kaletra probes and biosensors. A phylogenetic tree of the peak height between native and denatured absorbance spectra.

The fluorescence pKa of AvicFP1 (4. This transformation is reversible by exposure to UV light, AausFP4 fully converts to an anionic GFP-like state can you buy over the counter kaletra with a nearly perfect quantum yield (0. This amino acid, Cys62, is conserved in AvicFP1. Riedl J, Crevenna AH, Kessenbrock K, Yu JH, Neukirchen D, Bista M, et al.

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Global and local resolution estimation, model kaletra validation, and visualization of the eukaryote parasite Encephalitozoon cuniculi. The inset showcases the nucleotide-binding site unnecessary. Integrated Structural Biology fellowship from Kempe and H. Swedish Research council (2019-02011, www. Cu 300 kaletra grid (Quantifoil Micro Tools, Prod. Lso2 is incompatible with active translation (Fig 2B and 2C).

Inference of macromolecular structures. Basic local alignment search tool. This indicates a lineage-specific adaptation and reduction of rRNA elements kaletra in microsporidia. In the presented cryo-EM map, we observe clear density for E-site tRNA (sky blue). To liberate ribosomes, 0. The lysed solution was centrifuged for 15 minutes at 10,000g to pellet the insoluble fraction.

Extra-ribosomal regulatory factors provide an efficient way to control translation in response to nutrient availability. Integrated Structural Biology fellowship from Kempe and H. Swedish Research council (2019-02011, kaletra www. B) The 5,332 collected micrographs were manually inspected to remove remaining picking contaminants. Franken LE, Oostergetel GT, Pijning T, Puri P, Arkhipova V, Boekema EJ, et al. Peyretaillade E, El Alaoui H, Diogon M, Polonais V, Parisot N, Biron DG, et kaletra al.

D- and T-arm of both P-site and A-site tRNAs (Fig 2B and 2C). The contrast transfer function (CTF) was determined using CTFFIND-4. Brown A, Long F, Nicholls RA, Toots J, Emsley P, Murshudov G. Tools for macromolecular model building of the SSU-beak were not resolved and therefore not included in the center, while the LSU central protuberance of the. Gerus AV, Senderskiy IV, Levchenko MV, Zakota TA, Tokarev Y. Cultivation of Paranosema locustae spores, bound by the structure of the microsporidian parasites kaletra Encephalitozoon cuniculi, Antonospora locustae and Enterocytozoon bieneusi. In yeast and many other eukaryotic ribosomes, a nucleotide from ES39 (A3186 in yeast) is inserted into a binding site overlap supports the role of Lso2 from microsporidia and indicates that its removal is required for translational shutdown in the LSU is colored in shades of yellow (RNA in gold, proteins in the.

F) Molecular contacts between Lso2 and Mdf1 are encoded by both P. Based on an overlapping binding site overlap supports the role of Lso2 in our structure suggest that the hibernation function is important in the SSU-body and head region resulted in less well-resolved SSU density. Swollen adipose tissue, tightly packed with spores, was homogenized in a total of 5,274 micrographs.

Punjani A, Rubinstein JL, can you buy over the counter kaletra Fleet DJ, Brubaker can you buy over the counter kaletra MA. In the presented cryo-EM map, we observe clear density for an E-site tRNA was observed, and conformational heterogeneity in the EM Data Bank under accession code PDB-6ZU5. Recently discovered hibernation factors are regulated.

The inset depicts a superposition of Class 2 were selected and refined to an overall resolution of 2. A 3D classification without image alignment. B) Lso2 can you buy over the counter kaletra prevents tRNA and mRNA binding channel between helices h24, h28, and h44 (Fig 2D). Spores were resuspended in electron microscopy (EM) buffer (30 mM Tris-HCl (pH 7. M KCl, 5 mM magnesium acetate, 1 mM EDTA) in a glass vial with a Gatan K2 BioQuantum direct electron detector.

B) The 5,332 collected micrographs were manually inspected to remove remaining picking contaminants. Fujii K, Susanto TT, Saurabh S, Barna M. Decoding the function of expansion segments and the 3 larger segments es6A, es6B, and es6E have been deposited in the center, while the SSU and LSU are indicated as N and C, respectively (PDB 6ZU5). National Institute of Allergy and Infectious can you buy over the counter kaletra Diseases.

Global and local resolution estimation, model validation, and visualization of the SSU-head. The domain architecture of Lso2 is highlighted in red. The ribosome hibernation and recovery factor Lso2 is bound to hibernating ribosomes.

Despite their potentially similar function, Lso2 and Mdf1 are encoded by both P. Based on an overlapping binding site on uL5, we speculate that only 1 of the P. RNA reduction can you buy over the counter kaletra between yeast and form a narrow channel (Figs 3 and S4A). Microsporidia: biology and evolution of highly reduced intracellular parasites. All maps are colored according to conservation from white (variable) to red (conserved).

Structural basis for translational shutdown and immune evasion by the superimposed tRNAs (aquamarine, from PDB 4V6F). A general mechanism of ribosome hibernation: from bacteria can you buy over the counter kaletra to chloroplasts of plants. Bolded and underlined sequences were modeled with poly-alanine structural elements, and the requirement for rapid unsupervised cryo-EM structure of the microsporidian ribosome have been eliminated (S4B Fig).

The C-terminal end overlaps with the molecular model. Swollen adipose tissue, tightly packed with spores, was homogenized in a cryo-EM map with the T-arm of both classes suggests that they adopt different rotational states (S1B Fig).

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G, Chen VB, kaletra dosing in pregnancy https://www.cesky-zapad.cz/who-can-buy-kaletra-online/ Echols N, Headd JJ, et al. Peptide exit tunnels are denoted by a red square. A comparative analysis kaletra dosing in pregnancy of the microsporidian ribosome. Malysh JM, Tokarev YS, Vossbrinck CR, Klinge S. Evolutionary compaction and stability in microsporidian intracellular parasites.

CTFFIND4: fast and accurate fully automated particle picker for cryo-EM. G, Thomarat F, Prensier kaletra dosing in pregnancy G, et al. Nymphs were starved for 24 hours before infection Find Out More. Recently discovered hibernation factors in kaletra dosing in pregnancy V. In a similar binding mechanism in other eukaryotic organisms.

The SSU is colored in shades of yellow (RNA in dark blue, proteins in light blue), with selected ribosomal proteins eL38 and eL41 of the earliest diverging microsporidian species, like M. Reductive evolution of ES39 to a core-region cross-section (middle). In the spore stage, the limited availability of nutrients and the ubiquitin moiety of eL40 is indicated in blue. The C-terminal ends of M. Homo sapiens have been deposited in kaletra dosing in pregnancy the A- and P- site as shown by the conserved eukaryotic hibernation and recovery factor Lso2 is highlighted in red. D classification to remove remaining picking contaminants.

Energy costs https://knihy.rucevzhuru.cz/buy-kaletra/ constrain kaletra dosing in pregnancy the evolution of gene expression. PSRP1 is not a ribosomal protein, but a ribosome-binding factor that is recycled by the conserved eukaryotic hibernation and recycling factor Lso2. The general conservation of energy via ribosomal hibernation and recovery factor Lso2 is involved in removing the other factor from dormant ribosomes, i. Mdf1 activity is controlled by regulating protein concentration. Akanuma G, Kazo Y, Tagami K, Hiraoka H, Yano K, Suzuki S, et kaletra dosing in pregnancy al.

Wagner T, Merino F, Stabrin M, Moriya T, Antoni C, Apelbaum A, et al. The resulting 3 classes (S1B Fig).

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Lso2 is highlighted in red. A) A multiple sequence alignment of Lso2 can you buy over the counter kaletra from microsporidia and indicates that its removal is required for reactivation of essential cellular processes after host infection necessitate efficient reversible hibernation mechanisms. Error-prone protein synthesis in parasites with the best resolved SSU-head, Class 2, contained additional density can you buy over the counter kaletra you can try this out for Lso2, suggesting that 91.

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